119th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists Ⅰ
Room No. 303호
Time THU 09:28-:
Code ANAL1.O-7
Subject Super-Localization of Immunoplasmonic Particle for Ultra-Sensitive Detection of Alpha-Fetoprotein on Nanobiosensor by Scattering-Based Super-Resolution Microscopy
Authors 안수진, 강성호1,*
경희대학교 일반대학원 화학과, Korea
1경희대학교 응용화학과, Korea
Abstract Immunoplasmonic particles were super-localized within highly specific region for sensitive detection of alpha-fetoprotein (AFP) with total internal reflection scattering super-resolution microscopy (TIRS-SRM). 20-nm silver nanoparticles were used as a nanoprobe to detect AFP molecules and 100-nm gold nanoisland (GNI, substrate) were selected as a substrate. Individual scattering signals and images of immunoplasmonic particle and GNI were acquired within evanescent field layer by TIRS-SRM using 405 nm and 635 nm illumination, respectively. The center coordinates of the Immunoplasmonic particles were determined within highly specific region (GNI) by applying 2D Gaussian fitting to point spread function. Limit of detection (LOD) of the developed method was 7.04 zM, which was at least 100 times lower than LODs obtained with the previously reports. The TIRS-SRM was demonstrated to be an effective tool to localize individual protein molecules within specific regions for distinguishing each signal and sensitive detection. The method was reliable and effective detection of disease-related protein molecules as diagnostics.
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