119th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists Ⅰ
Room No. 303호
Time THU 09:38-:
Code ANAL1.O-12
Subject Supersensitive Plasmonic Single Nanoparticle-based Cancer Antigen 125 Immunodetection by Dual-Mode Wavelength-Dependent Enhanced Dark-Field Microscopy
Authors 주소영, 강성호1,*
경희대학교 일반대학원 화학과, Korea
1경희대학교 응용화학과, Korea
Abstract A supersensitive immunodetection was achieved for the biomarker cancer antigen 125 (CA125), which was developed based on characterization of single-particle plasmonic scattering by dual-mode wavelength-dependent enhanced dark-field microscopy (EDFM-DM). For simultaneous dual-detection, an electron multiplying cooled charge-coupled device camera and color digital camera were used for qualitative and quantitative analysis, respectively, based on the dark-field scattering images. To prove the efficacy of EDFM-DM for the dectection of scattering signals, various size of different plasmon nanoparticles (i.e., gold nanoparticles, 5 nm, 12 nm, 20 nm, 100 nm and 250 nm; silver nanoparticles, 20 nm, 30 nm, 40 nm, 80 nm and 100 nm) were used as the detection tags and investigated with wavelength dependence of the light source, and the quantum efficiency of the electron-multiplying charge-coupled device camera, 40-nm silver nanoparticle (AgNP) was selected as an optimum fluorescence-free probe. CA125 ovarian cancer antigen was screened at single-molecule level and quantitatively analyzed by measuring the scattering signals of 40-nm AgNP on gold-nanodots arraychip with lowest possible LOD (4 μU/mL, S/N = 3) with a wide dynamic detection range of 4 μU/mL-80 U/mL (R = 0.9935), which was a 100-375,000-fold lower detection limit with a 100-100,000-fold wider dynamic range than previous detection methods. In addition, recovery was greater than 98% with the spiking of standard CA125 in human serum samples. Dual-mode EDFM-DM based optical property of the plasmon nanoparticle allowed us for a sensitive detection of disease related biomolecules at single-molecule level.
E-mail soyeongju@naver.com