119th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists Ⅰ
Room No. 303호
Time THU 09:32-:
Code ANAL1.O-9
Subject Real-Time In-Situ Nano-Carrier Quantification by Super-Resolution Microscopy in Live Single Cells
Authors Chakkarapani Suresh Kumar, 강성호1,*, 신재호1
경희대학교 화학과, Korea
1경희대학교 응용화학과, Korea
Abstract The extent of time-dependent subdiffraction-limit super-resolution imaging in single molecule tracking and localization has been a great asset in the biomedical field. Quantifying and assessing drug/gene- carrying nanoparticles at the cellular level was crucial in visualizing the nano-toxicity to hold good for drug delivering applications. In this work, a 50 nm drug carrier containing rhodamine B isothiocyanate dye was quantified, tracked, and assessed toxicity at HEK293, NIH3T3, and RAW 264.7 cells using direct stochastic optical reconstruction microscopy (dSTORM). Results suggest that the RAW 264.7 cells possess higher uptake efficiency of nano-carrier than HEK293 and NIH3T3 cells. Moreover, the nano-carrier was observed to break the biological barrier to be internalized within the nuclei of RAW 264.7 and HEK293 cells. Additionally, the micronuclei were detected more in RAW 264.7 cells than in other two cells suggesting disruption in usual chromosomal DNA breakage. A first ever combined approach of dSTORM, transcriptomics, and molecular biology paid way for assessing nano-carrier in three different cells at specific sites with precise sensitivity. The subdiffraction-limit super-resolution microscopy dSTORM resolves quantitative differences in the uptake efficiency of nano-carrier and assess internalized nano-carrier locality in live single cell.
E-mail sureshsrkmv@gmail.com