119th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists Ⅰ
Room No. 303호
Time THU 10:08-:
Code ANAL1.O-27
Subject Stability of N-glycans Found in Human Saliva using Nano LC Chip/Q-TOF MS
Authors 김범진, 문한태, 안현주*
충남대학교 분석과학기술대학원, Korea
Abstract For the past decades, saliva glycans hold the attention as a potential bio-signature in clinical and forensic field owing to its inherent advantages; easy access, simple storage, and non-invasive collection. Glycosylation, one of the most common PTMs, plays an important role in a wide range of biological processes. Saliva glycosylation could be altered in the physiological and pathological situations by diseases, external environment, and chemical materials. Although profiling of glycans in saliva for the discovery of potential biomarkers for inflammatory diseases and cancers has been actively performed, fundamental studies such as saliva stability and glycans variations between individuals have been rarely done. In this study, we have investigated variations of saliva N-glycans 1) between individuals (n=18), 2) by a single individual using saliva collected on different days (0, 1, 7, and 30 days), and 3) during the storage of saliva (0, 1, 2, 3, and 7 days) in room temperature. Saliva N-glycans were enzymatically released by PNGase F and then purified and enriched by PGC-SPE. Saliva N-glycans were fully characterized by nano LC chip/Q-TOF MS and MS/MS. Inter- and intra-individual and environmental variations were qualitatively and quantitatively examined through glycan compositions and abundances. This study may help design the experiments and evaluate the saliva glycan candidates for biomarker study.
E-mail kbj0131@gmail.com