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| Type |
Oral Presentation |
| Area |
Oral Presentation of Young Analytical Chemists Ⅰ |
| Room No. |
303호 |
| Time |
THU 09:42-: |
| Code |
ANAL1.O-14 |
| Subject |
Metabolic alteration induced by IDH1 R132H mutant of glioma revealed by LC-MS/MS |
| Authors |
황민지, 김민식*
경희대학교 응용화학과, Korea
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| Abstract |
Glioma is a type of brain tumor originating from tissues that bind and nourish neural tissue inside the brain and spinal cord. Most patients who are diagnosed with glioblastoma, the most common type of glioma have a median survival rate only for about 14 months, and only 3 to 5 percent of the patients are known to survive for more than 5 years [1][2]. Recently the R132H mutation of isocitrate dehydrogenase 1 (IDH1) were discovered in glioblastomas [3]. IDH1 is a metabolic enzyme that catalyzes the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate in the cytosol [4]. However, the IDH1 R132H mutant enzyme converts isocitrate to 2-hydroxyglutarate (2HG) instead that leads to a hypermethylated state of DNA and histones altering different gene expression in glioma [5]. The goal of this study is to quantitatively survey the metabolic profiles between normal IDH1 and mutant IDH1 R132H by LC-MS/MS. We found that a number of metabolites related to major metabolic pathways were altered with the IDH1 R132H mutation in glioma. This result can be used to find potential targets in glioma with the IDH1 R132H mutation. We expect that the comprehensive understanding of metabolic alteration induced by IDH1 R132H mutant of glioma may help the development of inhibitory drugs that may rectify the altered metabolic pathways by 2HG. |
| E-mail |
ghkdalswl00@naver.com |
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119th General Meeting of the KCS