119th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists Ⅰ
Room No. 303호
Time THU 09:50-:
Code ANAL1.O-18
Subject Evaluation of risk factors for non-alcoholic fatty liver disease using nanoflow UPLC-ESI-MS/MS
Authors 변슬기, 문명희*
연세대학교 화학과, Korea
Abstract Non-alcoholic fatty liver disease (NAFLD) is one of the most frequent cause of deaths worldwide, associated with obesity and insulin resistance due to unhealthy lifestyle. With excessive amount of fat accumulated on liver tissue, a spectrum of NAFLD encompasses a wide range of conditions ranging from simple steatosis to advanced cirrhosis. As a number of factors such as high cholesterol diet and inflammation are possible risk factors of NAFLD, the effect of various risk factors on hepatic lipid metabolism was evaluated in this study using nanoflow ultrahigh-pressure liquid chromatography electrospray-ionization tandem mass spectrometry (nUPLC-ESI-MS/MS). Lipids from hepatic tissue from rabbits grown under the conditions of healthy diet (C), inflammation by injecting carrageenan (I), high cholesterol diet (HC), and high cholesterol diet with inflammation by injecting carrageenan (HCI) were structurally identified based on collision-induced dissociation (CID) spectra obtained from nUPLC coupled with ion trap MS, followed by a targeted quantification of the identified lipids using nUPLC coupled with triple quadrupole MS in selective reaction monitoring (SRM) mode. Over 300 hepatic phospholipids, sphingolipids, and glycerols were analyzed and significant and dramatic changes were observed from HC and HCI groups but not I, indicating that intake of high cholesterol food altered hepatic lipidome significantly while inflammation did not.
E-mail c.byeon@gmail.com