119th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists Ⅰ
Room No. 303호
Time THU 09:54-:
Code ANAL1.O-20
Subject Analysis of prostate cancer-derived urinary exosomes by flow field-flow fractionation
Authors 양준선, 문명희*
연세대학교 화학과, Korea
Abstract Exosomes are extracellular vesicles with 20 to 100 nm in diameters and they are involved in intercellular communication by transporting biomolecules such as mRNA, proteins, and lipids. They exist in all kinds of body fluids including urine and blood. Recently, research on exosomes of body fluids has emerged as a crucial component in biomedical field as exosomes are reportedly potential biomarker candidates of many diseases. Among various types of exosomes, urinary exosomes are closely associated with a development of prostate cancer (PCa) and their sizes are different compared to healthy controls. Flow field-flow fractionation (FlFFF) was used in this study to fractionate urinary exosomes by their size. FlFFF is an elution-based method for separating sample components according to size by an increasing order of hydrodynamic diameters and as raw samples can be directly injected into an empty FlFFF channel, the separated sample components can be collected in their intact forms, which is one of a great advantage of using FlFFF. In this study, urinary exosomes from healthy controls and PCa patients, which were first isolated from fresh urine samples by ultracentrifugation, were separated by FlFFF and clear difference in size distribution between two groups was observed. Furthermore, size-separated urinary exosomes were collected for lipidomic analysis using nUPLC-ESI-MS/MS and different lipidomic profiles and variations from large- and small-sized exosomes were observed between the groups.
E-mail js0610@yonsei.ac.kr