|
|
| Type |
Oral Presentation |
| Area |
Oral Presentation of Young Analytical Chemists Ⅰ |
| Room No. |
303호 |
| Time |
THU 09:48-: |
| Code |
ANAL1.O-17 |
| Subject |
Top-down proteomic analysis of HDL from coronary artery disease patients using flow field-flow fractionation and mass spectrometry
|
| Authors |
이재현, 양준선, 문명희*
연세대학교 화학과, Korea
|
| Abstract |
Coronary artery diseases (CAD) refers to a condition in which coronary arteries become narrow by accumulation of cholesterols, fats, and other substances. Narrow arteries restrict blood flow to heart and may even cause a partial or complete blockage of arteries. High-density lipoprotein (HDL) has been reported to be associated with CAD of which few marker proteins are of interests. HDL is composed of various lipids and proteins including apolipoproteins and recent studies have reported that apolipoprotein A-I (apo A-1) in HDL undergoes oxidation during the development of CAD. As other types of proteins in HDL are highly likely to undergo changes, analysis of HDL-related proteins is necessary to understand the relationship between HDL and CAD more effectively.
Flow field-flow fractionation (FlFFF) is a separation technique based on size in a range of nano to micrometer and in this study, FlFFF was applied to separate the HDL from human plasma of CAD patients. A miniaturized asymmetrical FlFFF (AF4) channel was coupled on-line to electrospray ionization mass spectrometry (ESI-MS) for a high speed separation of lipoproteins and top-down proteomic analysis in order to investigate the perturbation in HDL-related proteins due to development of CAD.
|
| E-mail |
lgs3845@naver.com |
|
119th General Meeting of the KCS