119th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists Ⅰ
Room No. 303호
Time THU 09:56-:
Code ANAL1.O-21
Subject Qualitative analysis of lipoproteins from patients with mild cognition impairment and Alzheimer’s disease by asymmetrical flow field-flow fractionation and nUPLC-ESI-MS/MS
Authors 김산하, 문명희*, 양준선
연세대학교 화학과, Korea
Abstract Lipoproteins are carrier molecules that transport lipids and cholesterol in the bloodstream. Low levels of high-density lipoprotein (HDL) and high levels of low-density lipoprotein (LDL) in plasma are known to be related with the development of various types of diseases. Therefore, it is important to study lipoproteins at the molecular level to understand its metabolism and relationship to disease progression. Alzheimer’s disease is the most common form of dementia worldwide, taking up around seventy percent of dementia cases. A beta-amyloid, a cause of Alzheimer’s disease, is a toxic protein that kills brain neurons as its number increases. As a few studies have reported that beta-amyloid accumulates when HDL is low and LDL is high in plasma, a separate profiling of lipids from HDL and LDL is necessary to understand how the lipid metabolism within each class of lipoprotein is altered when Alzheimer’s developed. In this study, HDL and LDL from of pooled plasma samples of human controls, patients with mild cognition impairment, and Alzheimer’s disease patients were fractionated by asymmetrical flow-field flow fractionation (AF4) and lipids from the collected fractions of HDL and LDL were extracted. Using nanoflow ultrahigh-pressure liquid chromatography-electrospray ionization-tandem mass spectrometry (nUPLC-ESI-MS/MS), the extracted lipids were structurally identified based on collision-induced dissociation for qualitative analysis and the identified lipids are going to be selectively quantified to evaluate the difference between three groups.
E-mail sanhakim92@yonsei.ac.kr