119th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists Ⅰ
Room No. 303호
Time THU 10:00-:
Code ANAL1.O-23
Subject 3D topological studies of antimicrobial peptides with enhanced activities derived from Lactophoricin
Authors 김지선, 정지호, 김용애*
한국외국어대학교 화학과, Korea
Abstract Since wide spread of antibiotic resistance bacterial pathogens have been prevalent it is important to investigate new class of antimicrobial molecules, antimicrobial peptides (AMPs). AMPs are usually relatively short positively charged polypeptides and exhibiting amphipathic character. Despite significant differences in their structures, all AMPs discovered share the ability to interact with cellular membranes, thereby disrupting membrane organization. Lactophoricin (LPcin), a cationic amphipathic peptide consists of 23-mer peptide, was currently utilized as the framework to design the novel analogs and study the effect of peptide hydrophobicity/hydrophilicity, amphipathicity on antimicrobial activities. LPcin analogs of 11 were designed and modified to enhance antibacterial activity using conservative sequence change. Three peptides were selected among LPcin analogs via results of bacterial killing and growth inhibition assays. We successfully overexpressed LPcin analogs in the form of fusion protein in Escherichia coli and purified them from the cell extracts with many biophysical techniques. In order to elucidate the structure-antibiotic activity relationships of the peptides, we studied the correlation between the modified conformation of LPcin analogs and their antimicrobial activity using various spectroscopic methods. The structural calculations of LPcin analogs using Discovery Studio 2016 were also used to refine the orientational information of 3D structure and topology based on 2D SAMPI4 solid-state NMR spectra. And we will also present the optimized design, construction, and good efficiency of a home-built 800 MHz (NB) narrow-bore 1H-15N solid-state NMR probe for solid-state NMR studies of antimicrobial peptides.
E-mail cucu860@daum.net