119th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists Ⅰ
Room No. 303호
Time THU 10:10-:
Code ANAL1.O-28
Subject Characterization of Glycan Signatures of Human Saliva using Nano LC Chip/Q-TOF MS
Authors 문한태, 김범진, 안현주*
충남대학교 분석과학기술대학원, Korea
Abstract In recent years, saliva has been received attention in forensic field. Saliva encountered at crime scene is one of the most significant evidence and thus, the identification and differentiation of saliva from other human and non-human fluids is an essential prerequisite prior to further crime investigation. However, conventional methods including enzymatic and chemical amylase test to determine saliva have lack of specificity due to cross-reactivity with other fluids. Glycoproteins are the major components of human saliva. Therefore, glycosylation in saliva can be used as a potential candidate of signature of human saliva. Herein, we have explored the possibility of nano-LC/MS-based glycomic approach to identify and differentiate bio-signature between human saliva and other fluids. Briefly, N-glycans in human saliva (7 males and 11 females) were enzymatically released and enriched by PGC-SPE. Human saliva N-glycans were profiled by nano LC-PGC chip/Q-TOF MS and –MS/MS. Saliva N-glycans displayed a heterogeneous mixture of neutral and acidic components and the most of them were decorated with fucose (> 50%). Interestingly, highly fucosylated glycans containing from tetra to octa fucose (Hex5-7HexNAc4-6Fuc4-8) were observed in high abundance in human saliva. To explore human saliva-specific glycans, human saliva was compared with other fluids such as human serum, human milk, and animal saliva (rat and bovine) we conclusively determined that highly fucosylated N-glycans were saliva-specific molecules found in only human saliva. Of them tetra fucosylated bi-antennary (Hex5HexNAc4Fuc4) and tri-antennary (Hex6HexNAc5Fuc4) glycans were present in all human saliva.
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