|
|
| Type |
Oral Presentation |
| Area |
Oral Presentation of Young Analytical Chemists Ⅰ |
| Room No. |
303호 |
| Time |
THU 10:20-: |
| Code |
ANAL1.O-33 |
| Subject |
A Sandwich-type HIV-1 p24 immunoassay using ICP-MS with metal-doped nanoparticles |
| Authors |
김찬미, 김인애1, 김은지, 주나영, 임흥빈*
단국대학교 화학과, Korea
1(주)에스엘에스 유전체의학연구소, Korea
|
| Abstract |
A virus is a type of non-cellular infectious agent that replicates inside the host cells of other organisms. Human Immunodeficiency Virus (HIV) causes acquired immunodeficiency syndrome (AIDS) and HIV-1 p24, a component of the HIV particle capsid, is an antigen detected in infected patients’ blood. For detection of HIV-1 p24, the sandwich-type conjugates employing metal-doped magnetic nanoparticles (MNPs) and metal/dye-doped silica nanoparticles (SNPs) were produced by immunoreaction and the concentration of the target was determined by ICP-MS in this work. For this, both Cs-doped Fe3O4 MNPs and Gd/RhBITC-doped SNPs for target extraction and tagging as a probe, respectively, were synthesized and immobilized with the antibodies of HIV-1 p24. Feasibility of quantification was shown from the calibration curve plotted the intensity ratio of Gd/Cs vs. the concentration of target. For future work, this method will be applied to real samples and multiplex detection of other viruses. |
| E-mail |
whflsepd@naver.com |
|
119th General Meeting of the KCS