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| Type |
Oral Presentation |
| Area |
Oral Presentation of Young Analytical Chemists Ⅰ |
| Room No. |
303호 |
| Time |
THU 10:22-: |
| Code |
ANAL1.O-34 |
| Subject |
Microwave-assisted week acid hydrolysis of proteins using hydrochloric acid generating serial detachment of amino acids |
| Authors |
백지현, 김정권*
충남대학교 화학과, Korea
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| Abstract |
Microwave-assisted week acid hydrolysis of proteins has been developed as an alternative approach for protein sequence analysis. Microwave-assisted weak acid hydrolysis of proteins can be performed with a small amount weak acid (e.g. 2%) such as formic acid, acetic acid or phosphoric acid in a microwave oven for an hour incubation. Weak acid hydrolysis cleaves exclusively C-terminal of aspartic acid. This method is simpler and faster than using a protease such as trypsin. It was reported that 3 M HCl hydrolyzed a protein into polypeptide ladders with varying sizes of up to molecular mass of the protein. Here we tried to truncate N-terminal of aspartic acid with small amount of hydrochloric acid for microwave-assisted acid hydrolysis after microwave-assisted week acid hydrolysis of myoglobin. The 0.5 ~ 1% HCl hydrolysis of C-terminal truncated myoglobin peptides provided major peptides cleaved selectively at the N-terminal of aspartic acids. The cleaved peptides was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, where the peptides cleaved with the N-terminal of aspartic acid were observed as major peaks while the peptides with the C-terminal cleavage of aspartic acid were observed as minor peaks. |
| E-mail |
paek5456@naver.com |
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119th General Meeting of the KCS