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| Type |
Oral Presentation |
| Area |
Oral Presentation of Young Analytical Chemists Ⅰ |
| Room No. |
303호 |
| Time |
THU 10:32-: |
| Code |
ANAL1.O-39 |
| Subject |
Using Electromembrane Extraction for Determination of NSAIDs by HPLC-UV |
| Authors |
김진문, 조현우1, 명승운*
경기대학교 화학과, Korea
1경기대학교 자연과학화학과, Korea
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| Abstract |
The electromembrane extraction(EME) is one of the liquid phase microextraction(LPME) which can extract and concentrate the analyte from aqueous matrices such as urine and plasma effectively. This method was applied to analysis of four Non-steroidal anti-inflammatory drugs(NSAIDs) in human urine samples. The parameters such as pH of acceptor, stirring speed, voltage, support liquid membrane(SLM), and extraction time were optimized. Simultaneous chromatographic separation of four NSAIDs by HPLC-UV/Vis system was achieved on an Eclipse XDB-C18 (4.6 mm i.d. × 150 mm length, 5 ㎛ particle size) column using gradient elution with 0.1 % formic acid and methanol. 0.1 % formic acid and methanol start to 30:70 and after 5minutes, compositon is changed to 15:85, finally, changed to 5:95 after 5minutes. After the extraction, 5 ㎕ of extract was directly injected into the HPLC-UV system. |
| E-mail |
jm12355@naver.com |
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119th General Meeting of the KCS