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| Type |
Oral Presentation |
| Area |
Oral Presentation of Young Analytical Chemists Ⅰ |
| Room No. |
303호 |
| Time |
THU 10:36-: |
| Code |
ANAL1.O-41 |
| Subject |
Fabrication and Application of the Microfluidic Devices Designed to Simulate HET-CAM Test |
| Authors |
Tian Tian, 조수진, 이석우*
공주대학교 화학과, Korea
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| Abstract |
In this presentation, we will describe the development and application of a simple microfluidic device for in vitro irritation tests of cosmetics. The device was fabricated with a three-compartment diffusion system to mimic the HET-CAM system. HUVEC (Human Umbilical Vein Endothelial Cells) was cultured in the three compartments and the tested substances were injected to the reservoir and diffused through the tiny microfluidic channels. The viability of cells in each compartment was monitored by an optical microscope. The IS (irritation score) values were evaluated based on the viability of cells as a function of time when the cells were exposed to the tested substances. Three kinds of cosmetic materials were tested with microfluidic systems, and the results were compared with those of the HET-CAM test to validate the in vitro irritation test system. |
| E-mail |
jisanrhee@kongju.ac.kr |
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119th General Meeting of the KCS