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| Type |
Oral Presentation |
| Area |
Oral Presentation of Young Analytical Chemists Ⅱ |
| Room No. |
303호 |
| Time |
FRI 09:18-: |
| Code |
ANAL2.O-6 |
| Subject |
SERS-based immunosensor for the highly sensitive detection of mycotoxin using three-dimensional nano-pillar array chips |
| Authors |
WANG XIAOKUN, 주재범1,*
한양대학교 바이오나노학과, Korea
1한양대학교 생명나노공학과, Korea
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| Abstract |
Mycotoxins such as ochratoxin A, aflatoxin B1, fumonisin and others are well-known dangerous food contaminants that usually occur in trace amounts from nanograms to micrograms per gram of food. Due to their teratogenic and carcinogenic properties, mycotoxins present a serious hazard to human and animal health. The conventional detection methods such as high-performance liquid chromatography, mass spectroscopy and ELISA are extensively used for their quantitative analysis but they are rather expensive or time consuming; therefore, a new detection technique is still needed for the rapid and sensitive detection of Mycotoxins. For this purpose, we designed a novel SERS-based immunosensor for mycotoxin detection by combining a three-dimensional (3D) nano-pillar array chip with a surface-enhanced Raman scattering (SERS) detection technique. Upon target mycotoxins and mycotoxins antibody introduction, a competitive-type immunoreaction was implemented between the target and the immobilized mycotoxins-BSA on the 3D nano-pillar array chip. After this reaction, secondary antibodies conjugated on SERS nanotags were captured on the 3D nano-pillar array chip surface, forming a confined 3D plasmonic field, leading to the enhanced electromagnetic field on the 3D pillar surface. This work is expected to be a potentially useful tool for the rapid and sensitive detection of various mycotoxins. |
| E-mail |
wxkun8712@hotmail.com |
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119th General Meeting of the KCS