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| Type |
Oral Presentation |
| Area |
Oral Presentation of Young Analytical Chemists Ⅱ |
| Room No. |
303호 |
| Time |
FRI 09:44-: |
| Code |
ANAL2.O-19 |
| Subject |
A SERS-based magnetic immunoassay of fraction 1 antigen for Yersinia pestis utilizing integrated droplet microfluidics |
| Authors |
최남현, 주재범1,*
한양대학교 바이오나노공학과, Korea
1한양대학교 생명나노공학과, Korea
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| Abstract |
The combination of surface-enhanced Raman scattering (SERS)-based detection technique with lab-on-a-chip platform provides an ideal mechanism for achieving rapid, sensitive and reproducible assay of specific biomarkers in solution. An additional benefit of such platform is the use of minimal sample volumes and low analyte concentrations. We recently reported a SERS-based wash-free magnetic immunoassay technique using magnet-embedded droplet microfluidics, and its application to clinical diagnostics. In this platform, the assay could be performed without any washing process by separating immunocomplexes from the supernatant solution using a magnetic separation. Nonetheless, this methodology still limited in assay because the channel structure for reagents injection and mixing is unified in a channel. To resolve this problem, a new class of microdroplet device was designed and fabricated for a sequential immunoassay processing in an automatic manner. This device is composed of six distinct compartments including droplets generation, mixing, merging, isolation, separation, and detection. Herein, we report a novel SERS-based droplet microfluidic device for fully automatic immunoassay in the microfluidic channel. For the proof-of-concept, the device was applied to the detection of the fraction 1 antigen of plague marker which is well known as an important anti-phagocytic antigen of Yersinia pestis. |
| E-mail |
choi.namhyun@gmail.com |
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119th General Meeting of the KCS