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|
| Type |
Oral Presentation |
| Area |
Oral Presentation of Young Analytical Chemists Ⅱ |
| Room No. |
303호 |
| Time |
FRI 09:50-: |
| Code |
ANAL2.O-22 |
| Subject |
Development of advanced proteome-to-genome mapping technologies and application to proteogenomic characterization of early onset gastric cancer
|
| Authors |
문동기, 이한겨레, 이상원*
고려대학교 화학과, Korea
|
| Abstract |
Next-generation sequencing and MS-based proteomics have been increasingly adopted as powerful technologies for characterizing cancer genome and proteome. Also there have been endeavors for integrating genomic and proteomic data to achieve comprehensive understandings of cancer. To this end, sensitive, extensive, and quantitative profiling of proteome are needed. In this study, advanced proteome-to-genome mapping (P2G) technologies were developed, including standardized proteome sample preparation procedure, serial enrichment of PTMs, highly sensitive LC-MS/MS analysis platform, and improved post-experimental peptide identification tool. Developed P2G mapping technologies were applied for characterizing proteome of early onset gastric cancer. Global proteome, phosphoproteome and N-glycoproteome profiling were performed for paired tumor and adjacent normal tissues of 80 patients. Obtained proteome profiles were integrated with genomics data (e.g. exome and mRNA data), which provided additional knowledges on cancer biology that were not obtainable by genomic data only. |
| E-mail |
cacagong@naver.com |
|
119th General Meeting of the KCS