119th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists Ⅱ
Room No. 303호
Time FRI 09:52-:
Code ANAL2.O-23
Subject Influence of sedimentation on the cellular association and endocytosis mechanisms of silver nanoparticles: Flow cytometry and ICP-MS study
Authors 심유진, HA KIEU MY1,*, 윤태현2,*
한양대학교 화학과 , Korea
1한양대학교 화학과, Vietnam
2한양대학교 화학과, Korea
Abstract Recently, nanomaterials have attracted increasing interest and widely used in many applications, such as nanomedicine as well as consumer products. However, their accumulation in the environment raises concerns on the potential hazards of nanomaterials on biological and environmental systems.1 Silver nanoparticles (Ag NPs) are widely used as antimicrobial agents, laundry detergents, wall paint and clothing such as underwear and socks.2 Notwithstanding the increasing use of Ag NPs in consumer products and potential risk of human exposure, there is no quantitative understanding on their cellular association mechanism and resultant toxicity .3 To better understand these phenomena, it is prerequisite to develop measurement method and perform quantitative analysis of intracellular Ag NPs.4 The purpose of this study is to investigate the influence of nanoparticle sedimentation on their cellular association and related endocytosis mechanism, when A549 cells were exposed to Ag NPs. We have measured the dispersion stability, hydrodynamic sizes, and surface charges of Ag NPs using UV-Vis spectroscopy, dynamic light scattering (DLS) and Zeta-potential. We also performed flow cytometry (FCM) and inductively coupled plasma – mass spectrometry (ICP-MS) measurements to quantify cellular Ag NPs under various exposure conditions. Inhibitor assays on endocytosis mechanisms were also conducted and confirmed with flow cytometry (FCM) and bio-transmission electron microscopy (bio-TEM), which provided us useful insight on the cellular uptake mechanisms of Ag NPs in A549 cells.
E-mail youckdduf@gmail.com