119th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists Ⅱ
Room No. 303호
Time FRI 10:12-:
Code ANAL2.O-33
Subject Upconversion Nanoparticles Functionalized with Novel Amphiphilic Polymers for Enhanced Biocompatibility and Bioimaging of KB Cells
Authors Salah Mahmoud Tawfik Ahmed, SHARIPOV MIRKOMIL1, B.T.Huy2, GerelkhuuZayakhuu3, 정종원4, 이용일4,*
창원대학교 화학과, Egypt
1창원대학교 화학과, Uzbekistan
2창원대학교 화학과, Vietnam
3창원대학교 화학과, Mongolia
4창원대학교 화학과, Korea
Abstract We have synthesized amphiphilic alginate (Al-NH-PEG-NH2 and Al-NH-PEG-NH-FA)as the novel ligands to functionalize upconversion luminescent surface. Upconversion nanoparticles (NaLuGdF4: Yb,Er,Cr) were capped with a thin polymer shell by replacing the oleate ligand of hydrophobic UCNPs by multidentate carboxylates of amphiphilic alginate polymers. Using amphiphilic alginate polymers as the surface ligands not just enhances the stability and biocompatibility of the UCNPs under physiological conditions but also displayed enhanced upconversion luminescent intensity. It was found that a maximum upconversion luminescence enhancement of 20-fold was obtained in comparison with the hydrophobic UCNPs. In order to demonstrate the targeted bioimaging capability of the functionalized-UCNPs, two cell lines (KB and HeLa) were employed for the investigations on the basis of their different folate receptor expression level. The results illustrated that the UCNP-Al-NH-PEG-NH-FA was specific in targeting KB cell lines more than HeLa cell lines under an excitation wavelength of 980 nm. The cell viability of two types of cells incubated with high concentration (300 µg•mL-1) of functionalized-UCNPs still remained above 85% after 24 h. The formation of stable water-dispersible UCNPs with enhanced emission, together with their amphiphilic and biocompatible polymer coating, is promising for building multifunctional nanostructures for cell imaging, therapy, and drug delivery.
E-mail salahtwfk85@yahoo.com