119th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists Ⅱ
Room No. 303호
Time FRI 10:14-:
Code ANAL2.O-34
Subject Ultrasensitive fluorescence detection of heparin using CdTe quantum dots coated with amphiphilic polythiophenes
Authors 심지민, Salah Mahmoud Tawfik Ahmed1, 이용일*
창원대학교 화학과, Korea
1창원대학교 화학과, Egypt
Abstract A new strategy for the detection of heparin is developed by utilizing quantum dots (QDs) coated with amphiphilic polythiophenes. The amphiphilic polythiophenes (PTPQ2000)-coated CdTe QDs were synthesized by in situ polymerization of modified thiophene on the surface of CdTe QDs. The PTPQ2000-coated CdTe QDs has a tremendously enhanced fluorescence and improved biocompatibility compared to the uncoated CdTe QDs. The PTPQ2000-coated CdTe QDs was successfully utilized to detect heparin. The emission of PTPQ2000-coated CdTe QDs is found to be quenched upon addition of the polyanionic heparin, due to electron transfer via electrostatic and/or hydrogen bonding interactions between heparin and PTPQ2000-coated CdTe QDs. The fluorescence intensity quenched with the increase of heparin and a good linear relationship was observed for heparin detection in buffer solution and the limit of detection (LOD) is 0.75 nM. The selectivity results of the fluorescence assay revealed that our system displayed excellent fluorescence selectivity towards heparin over its analogues. This fluorescence "switch of" assay for heparin is label-free, less toxic, sensitive and selective, which can be used to detect heparin in biological systems.
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