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| Type |
Oral Presentation |
| Area |
Oral Presentation of Young Analytical Chemists Ⅱ |
| Room No. |
303호 |
| Time |
FRI 10:16-: |
| Code |
ANAL2.O-35 |
| Subject |
Phospholipase A2-responsive UCNPs-loaded Phosphate Micelles for Prostate Cancer Cells Imaging |
| Authors |
SHARIPOV MIRKOMIL, Salah Mahmoud Tawfik Ahmed1, GerelkhuuZayakhuu2, 이용일3,*
창원대학교 화학과, Uzbekistan
1창원대학교 화학과, Egypt
2창원대학교 화학과, Mongolia
3창원대학교 화학과, Korea
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| Abstract |
Rare-earth upconversion nanoparticles (UCNPs) have been proposed as biological luminescent labels for the bioimaging application. However, their toxicity, low dispersion, and low selectivity call into question their suitableness in nanomedicine. Herein, UCNPs-loaded phosphate micelles formed from the secreted phospholipase A2 (sPLA-2) enzyme cleavable phosphate micelles to deliver UCNPs directly to prostate cancer cells has been reported. These phosphate micelles were designed from biocompatible materials. Through a dye micellization method, the CMC value of micelles was determined to be 6.38x10-4M. The activity of (sPLA-2) enzyme toward the synthesized micelle was investigated and confirmed using LC-MS. TEM results showed that micelles have a size ranged between 50 to 80 nm; whereas UCNPs loaded micelles have from 60 to 100 nm. Bio-imaging experiments conducted on KB, HeLa, and 22Rv1 cell lines confirmed the selectivity of UCNPs-loaded micelles toward 22Rv1 cell line. Moreover, the non-selective binding of UCNPs towards cells with low expression of PLA-2 was significantly reduced and MTT assay results confirmed the biocompatibility of micelles. |
| E-mail |
mirkosharipov@gmail.com |
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119th General Meeting of the KCS