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| Type |
Oral Presentation |
| Area |
Oral Presentation of Young Analytical Chemists Ⅱ |
| Room No. |
303호 |
| Time |
FRI 10:34-: |
| Code |
ANAL2.O-44 |
| Subject |
Glycan Heterogeneity Differentiation Using Lectin-based SDC Separation and Glycopeptide level SDC Separation
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| Authors |
정미선, 조원련*
원광대학교 바이오나노화학부, Korea
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| Abstract |
Previous work in this group showed displacement phenomena in lectin affinity chromatography in glycoprotein level using LEL Lectin-based SDC Separation. However, the SDC can fail to differentiate between glycoforms of proteins with multiple glycosylation sites when a high affinity glycan is conjugated to a protein at one site and a low affinity glycan at another site in the same protein. The high affinity glycan will dominate binding of the protein to the lectin column and the low affinity glycan will be unrecognized. This problem can be solved with the digestion of the glycoproteins using self-packed trypsin column and glycopeptide Lectin-based SDC Separation. Human pooled plasma were digested with the self-packed trypsin columns without chemicals. |
| E-mail |
misun9422@wku.ac.kr |
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119th General Meeting of the KCS