|
Type |
Poster Presentation |
Area |
Life Chemistry |
Room No. |
Exhibition Hall 2+3 |
Time |
10월 19일 (목요일) 11:00~12:30 |
Code |
BIO.P-239 |
Subject |
Live cell-based sensor for detecting biological signal molecules |
Authors |
Minhyeong Lee, hyunjin jeon, Youngeun Kwon* Department of Biomedical Engineering(BK21 plus), Dongguk University, Korea |
Abstract |
Signaling molecules mediate communication between cells in vivo and play key roles in regulating intracelluar function, metabolism, and cell fate. Detecting signaling molecules is significant for understanding these biological event. We present new tools to monitor signaling molecules using living cells, which is genetically encoded fluorescent sensor based on fluorescent trans-localization by intein-mediated reactions. The approach is based on the generation of functional signal peptide by intein-mediated splicing or cleavage reaction which is activated in response to external stimuli. First, we built a model system to use rapamycin-induced hetero-dimerization of FKBP and FRB. And then we constructed a live cell based sensing system to monitor the influx of Ca2+, an important intracellular signaling molecules related to various cellular events, using split-intein conjugated calmodulin and calmodulin binding domain. The sensing system successfully reported the change of intracellular Ca2+ levels induced by ionophore or histamine. Finally, the sensing of corticosterone, which affects various physiological responses, was also carried out using glucocorticoid receptor protein. This live cell-based sensing systems can be engineered to report various cellular events of interest in biologically relevant environment and can be used for various application such as drug screening and disease mechanism study. |
E-mail |
sksdlalsgud@gmail.com |
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