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| Type |
Poster Presentation |
| Area |
Organic Chemistry |
| Room No. |
Exhibition Hall 2+3 |
| Time |
10월 20일 (금요일) 13:00~14:30 |
| Code |
ORGN.P-265 |
| Subject |
Development of hemicyanine-based fluorescent probe for the detection of human NAD(P)H:quinone oxidoreductase (hNQO1) activity |
| Authors |
Jinju Lee, Min Hee Lee*
Department of Chemistry, Sookmyung Women's University, Korea
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| Abstract |
We developed a new hemicyanine-based fluorescent probe for detection and imaging of NAD(P)H:quinone oxidoreductase (hNQO1) in human cells. The hNQO1 enzyme is associated with various diseases and it is overexpressed in cancer cells. The probe consist of hemicyanine as a fluorogenic and colorimetric reporter, immolative linker, and trimethyl lock quinone as the hNQO1 enzyme sensitive part. This probe shows a broad absorption band at 480 nm and a very weak fluorescence intensity around 560 nm. However, upon reacting with the hNQO1, a significant increase in the fluorescence intensity and color change from yellow to red were observed in the physiological conditions. In addition, these changes were not observed in the case of other biologically relevant metal ions, redox species, and anions in the similar conditions. Moreover, we found that this probe can selectively detect the hNQO1 enzyme activity with a high specificity constant (kcat/Km = 4.05 ± 0.753 x 106 M-1 min-1), which was relatively higher value compared to other probes reported earlier. |
| E-mail |
naome0609@sookmyung.ac.kr |
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120th General Meeting of the KCS