120th General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Exhibition Hall 2+3
Time 10월 19일 (목요일) 11:00~12:30
Code BIO.P-242
Subject Sensitive and Specific MicroRNA Detection in a Single Neuronal Cell Using Atomic Force Microscopy
Authors ikbum Park, Joon Won Park1,*
Division of Integrated Biosciences and Biotechnolo, Pohang University of Science and Technology, Korea
1Department of Chemistry, Pohang University of Science and Technology, Korea
Abstract MicroRNAs (miRNA) are small, non-coding RNAs that control the translation of messenger RNAs, thereby regulating critical aspects of plant and animal development. Especially, specific miRNAs of brain have important roles in neuronal differentiation, development, plasticity and induced neurological disorders. Visualization of miRNAs has emerged as an important topic in biomedical fields. Therefore, accurate visualization of miRNAs at the single-cell level is of great interest due to their heterogeneity among cell population. Conventional methods widely used in the detection of miRNAs mainly include northern blotting, microarray, real-time RT-PCR and in-situ hybridization. Especially, In situ detection of intracellular miRNAs requires simple sample preparation, without cell lysis and RNA isolation, and provides additional information on the subcellular localization of miRNAs. However, conventional tools are not easy to detect high spatial localization and sensitive detection of miRNAs. Therefore, a new approach for miRNA detection tool is atomic force microscopy (AFM). AFM has been extensively used to observe interaction of molecules and manipulate biomolecules in the physiological conditions at the single-molecule level. Because AFM has high lateral resolution of sub-nanometer, the nanoscale distribution of individual molecules on the sample surface could be imaged. Here, we established an AFM-based miRNA quantification and distribution method with no requisite of labeling for modification of miRNAs. In this research, visualization of a specific miRNAs in a cultured neuronal cell is measured by force-based AFM and help to understand functions of miRNA in depth.
E-mail ibpark@postech.ac.kr