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| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Exhibition Hall 2+3 |
| Time |
10월 19일 (목요일) 11:00~12:30 |
| Code |
BIO.P-245 |
| Subject |
Microarrays on A Dendron-Modified Surface and Metal-Enhanced Fluorescence Nanoparticles Improving Sensitivity for MicroRNAs |
| Authors |
Soohyun Park, Joon Won Park*, Jwa-Min Nam1,*
Department of Chemistry, Pohang University of Science and Technology, Korea
1Division of Chemistry, Seoul National University, Korea
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| Abstract |
MicroRNAs(miRNAs) are small noncoding single-stranded RNAs(~22nt) that are involved in post-transcriptional modulation. The miRNA expression is associated with many diseases such as cancer, Alzheimer’s disease, myocardial disease. miRNA is 22mer shorter than general RNA, thus miRNA is amplified by PCR. In particular, the role of miRNAs in cancer has been extensively studied. miRNA analysis can be useful for early diagnosis or treatment of diseases.
Currently, Polymerase Chain Reaction (PCR) is a standard method to detect miRNA. However, it causes error in amplification process and analysis sensitivity is relatively low. In order to overcome the limitations of the conventional technology, I will improve the sensitivity more than 100 times using metal-enhanced fluorescence nanoparticles and microarray.
Here we suggest new approaches to supersensitive analysis of miRNA using metal-enhanced fluorescence (MEF) nanoparticles and microarray. To detect miRNA, we use S9.6 antibody that is DNA/RNA hybrid-specific mouse monoclonal antibody. This approach is expected to perform parallel analysis with detection sensitivity increased several hundred times more than the conventional methods.
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| E-mail |
parksoo@postech.ac.kr |
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120th General Meeting of the KCS