120th General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Exhibition Hall 2+3
Time 10월 19일 (목요일) 11:00~12:30
Code BIO.P-246
Subject Quantitative Analysis of Neuronal Proteins Using Atomic Force Microscopy
Authors Donggyu Lee, Joon Won Park1,*
Division of Integrated Biosciences and Biotechnolo, Pohang University of Science and Technology, Korea
1Department of Chemistry, Pohang University of Science and Technology, Korea
Abstract Proteins perform a vast array of functions within living organisms, including catalyzing metabolic reactions, DNA replication, responding to stimuli, and transporting molecules from one location to another. Detection of proteins of low expression level is frequently important, because a certain proteins exist at very low level during the early stages of cell development. In particular, neuronal proteins of the kind play a key role in the regulation of development and synaptic plasticity of neurons. Conventional methods to analyze proteins include western blotting, ELISA, and immunofluorescence. However, these tools suffer from poor limit of detection, and cannot visualize proteins in high lateral resolution. Therefore, atomic force microscopy (AFM) is a good candidate to overcome the hurdles. AFM is a powerful tool to show information about the distribution of the biomolecule. Using the mapping capability of AFM, it is possible to obtain a map the distribution of a specific protein in a nanometeric resolution. And we can count the copy number of protein in a sample solution. In the congress, we present our effort to demonstrate such capability of AFM.
E-mail lee2012@postech.ac.kr