120th General Meeting of the KCS

Type Poster Presentation
Area Analytical Chemistry
Room No. Exhibition Hall 2+3
Time 10월 19일 (목요일) 11:00~12:30
Code ANAL.P-166
Subject Forensic Platform for Identification of Human Saliva using MS-based Glycomics
Authors Hantae Moon, Bum Jin Kim, Hyun Joo An*
Graduate School of Analytical Science and Technology, Chungnam National University, Korea
Abstract In forensics, saliva found at crime scenes is one of the important evidences and thus, the identification of human saliva from other human fluids and non-human fluids, respectively is an essential prerequisite prior to further crime investigation. In previous study, we could determine that the degree and amount of highly fucosylated N-glycans were specific features to identify human saliva from other body fluids through MS-based glycomic approach. However, most saliva encountered at crime scenes has a trace amount and has been often found as a dry spot. For these reasons, forensic platform with high sensitivity for identification of human saliva is required. Herein, we have developed MS-based platform using glycomics and characterized N-glycans extracted from a trace amount of dried saliva. Briefly, dried saliva was prepared by spotting the saliva onto a protein saver card and drying at ambient temperature. N-glycans were enzymatically released by PNGase F from dried saliva spot (DSS) and enriched by solid phase extraction with a porous graphitized carbon cartridge. Then, saliva N-glycans were characterized by MALDI-TOF/TOF MS. N-glycans found in DSS showed a high correlation in terms of the number of glycans and their amount compared with N-glycans released from saliva fluid. Indeed, highly fucosylated N-glycans were also observed as a specific signature in DSS. We also examined technical validated reproducibility of N-glycan analysis based on DSS. This is the first study to apply a glycomic tool to forensic science.
E-mail htmoon90@gmail.com