|
Type |
Poster Presentation |
Area |
Analytical Chemistry |
Room No. |
Exhibition Hall 2+3 |
Time |
10월 19일 (목요일) 11:00~12:30 |
Code |
ANAL.P-166 |
Subject |
Forensic Platform for Identification of Human Saliva using MS-based Glycomics |
Authors |
Hantae Moon, Bum Jin Kim, Hyun Joo An* Graduate School of Analytical Science and Technology, Chungnam National University, Korea |
Abstract |
In forensics, saliva found at crime scenes is one of the important evidences and thus, the identification of human saliva from other human fluids and non-human fluids, respectively is an essential prerequisite prior to further crime investigation. In previous study, we could determine that the degree and amount of highly fucosylated N-glycans were specific features to identify human saliva from other body fluids through MS-based glycomic approach. However, most saliva encountered at crime scenes has a trace amount and has been often found as a dry spot. For these reasons, forensic platform with high sensitivity for identification of human saliva is required. Herein, we have developed MS-based platform using glycomics and characterized N-glycans extracted from a trace amount of dried saliva. Briefly, dried saliva was prepared by spotting the saliva onto a protein saver card and drying at ambient temperature. N-glycans were enzymatically released by PNGase F from dried saliva spot (DSS) and enriched by solid phase extraction with a porous graphitized carbon cartridge. Then, saliva N-glycans were characterized by MALDI-TOF/TOF MS. N-glycans found in DSS showed a high correlation in terms of the number of glycans and their amount compared with N-glycans released from saliva fluid. Indeed, highly fucosylated N-glycans were also observed as a specific signature in DSS. We also examined technical validated reproducibility of N-glycan analysis based on DSS. This is the first study to apply a glycomic tool to forensic science. |
E-mail |
htmoon90@gmail.com |
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