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| Type |
Symposium |
| Area |
Frontiers in Chemical Biology & Protein Chemistry |
| Room No. |
Room C311+C312 |
| Time |
FRI 09:25-: |
| Code |
BIO-2 |
| Subject |
Genetic incorporation of unnatural amino acids biosynthesized from simple starting materials |
| Authors |
HYUNSOO LEE
Department of Chemistry, Sogang University, Korea |
| Abstract |
The incorporation of unnatural amino acids (UAAs) with novel functional groups into proteins has expanded our ability to study protein structure and function. In this method, most components required to biosynthesize UAA-containing proteins are supplied by host cells into which the plasmids encoding an evolved tRNA/aminoacyl-tRNA synthetase (aaRS) pair and a target protein are introduced. The only additional component required is a UAA, which should be supplied in the growth medium. Many of these UAAs are expensive, and most of the biochemically interesting UAAs are not commercially available and have to be prepared via multistep synthesis. Therefore, new, less expensive and labor-intensive technologies for supplying UAAs to host cells are in demand, especially in a large-scale protein expression for pharmaceutical or industrial applications.
In this study, UAAs were biosynthesized by heterologous enzymes from simple starting materials, and the UAAs were directly incorporated into proteins in bacterial cells. This direct incorporation system showed efficient UAA incorporation with no incorporation of any natural amino acid and comparable protein yields with the genetic incorporation system using UAAs. In addition, biochemical applications of mutant proteins containing UAAs biosynthesized by this system showed its potential for protein conjugation and a large scale protein production for pharmaceutical and industrial applications. Finally, this work would provide an impetus for expansion of biosynthesis of unnatural amino acids to more challenging and interesting amino acids.
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| E-mail |
hslee76@sogang.ac.kr |
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120th General Meeting of the KCS