|
|
| Type |
Poster Presentation |
| Area |
Inorganic Chemistry |
| Room No. |
Exhibition Hall 2+3 |
| Time |
10월 19일 (목요일) 11:00~12:30 |
| Code |
INOR.P-36 |
| Subject |
Diiridium (III) Complexes as Emission Probes for G-quadruplex DNA and Cell Imaging |
| Authors |
Jinheung Kim*, Tikum Florence Anjong1
Chemistry Department of Nano-Science, Ewha Womans University, Korea
1Department of Chemistry and Nano Science, Ewha Womans University, Korea
|
| Abstract |
DNA G-quadruplex is a four stranded structure, derived from a guanine (G) rich DNA sequence. Though G-rich sequences have been demonstrated to form G-quadruplex structure in vitro, the existence of G-quadruplex structure in vivo is still controversial. Thus detecting G-quadruplex structure has great significance for cell proliferation, cancer research and drug development. Also, imaging of small organelles in the cell such as ER is very important, in understanding the structure and function of these organelles. Herein, two new dinuclear iridium (Ir) complexes bridged by a conjugate aromatic tppz ligand, were prepared. The DNA binding properties of both complexes where studied using single, double stranded and G-quadruplex DNA. Interestingly, the Ir complex demonstrated selectivity for G-quadruplex DNA over other control DNA which was observed by an enhancement in the phosphorescent spectra of the complex. In order to understand their possible interaction with lipid-rich organelles in live cells, the phosphorescent properties of both complexes were studied using liposomes which showed an increased in phosphorescent intensity upon the addition of liposomes with the probe showing a greater enhancement. We also confirmed that the Ir complexes could be used as probes in live cells. The probes were intensely stained in HeLa cervical cancer cells. Excitation with 488 nm laser showed red emission localized in the cytosol of cells. Cellular studies showed that the probes were located inside HeLa cells, but neither complex passed through the nuclear membrane. However, the complexes possessed superior photostability, indicating their role as good luminescent agents for imaging and tracking the endoplasmic reticulum (ER) in live cells. |
| E-mail |
jinheung@ewha.ac.kr |
|
120th General Meeting of the KCS