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|
| Type |
Poster Presentation |
| Area |
Physical Chemistry |
| Room No. |
Exhibition Hall 2+3 |
| Time |
10월 20일 (금요일) 13:00~14:30 |
| Code |
PHYS.P-99 |
| Subject |
Dynamics of Ligand Rebinding to Cytoglobin using Time-resolved Infrared Spectroscopy |
| Authors |
JuHyang Shin, Manho Lim*, CheongHa Lim1
Department of Chemistry, Pusan National University, Korea
1Pusan National University, Korea
|
| Abstract |
Femtosecond and nanosecond vibrational spectroscopy was used to probe the rebinding dynamics of CO to cytoglobin (Cgb) after photodeligation of the corresponding CO-bound protein in D2O buffer (pD = 7.4) at 273 K. When excited with a 532-nm photon, carbon monoxide dissociated immediately from cytoglobin (< 0.2 ps), ground state bleaching of CO bound to the cytoglobin has three conformations (A0, A1 and A3). Each conformation shows characteristic 13C−O stretching frequencies. The vibrational spectrum of CO in CgbCO described three gaussians at 1924 cm-1 (A0), 1899 cm-1 (A1) and 1884 cm-1 (A3) with a full width at half-maximum (FWHM) of 15 cm-1, 18 cm-1 and 12 cm-1. Ligand binding to Cgb is a multiphasic process with fast geminate rebinding taking place on the nanosecond timescales and slower biomolecular rebinding occurring on microsecond timescales. |
| E-mail |
dksvmflzk@gmail.com |
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120th General Meeting of the KCS