120th General Meeting of the KCS

Type Poster Presentation
Area Analytical Chemistry
Room No. Exhibition Hall 2+3
Time 10월 19일 (목요일) 11:00~12:30
Code ANAL.P-179
Subject LC-MS/MS determination and pharmacokinetic study of Sorafenib in rat and beagle plasma
Authors yoojeong yoon
Analytical Science center, Samyang Corporation, Korea
Abstract A rapid, sensitive and selective liquid chromatography/tandem mass spectrometry method (LC-MS/MS) has been developed and validated for the identification and quantification of Sorafenib in rat and beagle plasma using Sorafenib-d3 as an internal standard. Sample pretreatment involved simple protein precipitation (PPT). Chromatographic separation was carried out on a poroshell 120 EC-C18 column (3.0 × 50mm, 2.7 µm, Agilent) at 35 ℃ using an isocratic elution method with acetonitrile/0.1% formic acid in 10mM ammonium formate: 70/30 (v/v) at a flow rate of 0.2mL/min. Detection was performed using electrospray ionization in positive ion multiple reaction monitoring (MRM) mode by monitoring the ion transitions from m/z 465.3 → 252.2 (Sorafenib) and m/z 468.1 → 255.0 (internal standard). Calibration curves were linear in the concentration range of 5–5000 ng/mL. The overall precision and accuracy for all concentrations of quality controls and standards were better than 15%. The validated method was successfully applied to the pharmacokinetic study of Sorafenib in rat and beagle plasma. .
E-mail yoojeong.yoon@samyang.com