120th General Meeting of the KCS

Type Symposium
Area Physical Chemistry of Bioimaging and Biospectroscopy
Room No. Room 208+209+210
Time FRI 10:30-:
Code PHYS2-5
Subject Reversibly control of enzyme activity by pH-responsive DNA nanocages
Authors So Yeon Kim
Institute of Biomedical Engineering Research, Korea Institute of Science and Technology, Korea
Abstract To control enzyme activity regardless of the enzyme characteristics, cage-like DNA nanostructures have been used by encapsulating and releasing enzyme from the structure. However, these methods are irreversible, and thus have limitation in comparison with the reversible regulation observed in nature. Here, we present a method to covalently attach an enzyme inside of the DNA nanocage, whose structure can be reversibly switched by pH changes. We demonstrate that the enzyme accessibility to the surrounding environment, binding affinity to the corresponding antibody and even the enzyme activity can be reversibly controlled via pH dependent opening and closing of the DNA nanocage. Due to the size and pH response flexibility of DNA, our can be used as a broad-impact platform for controlling enzyme functions.
E-mail soyeonkim@kist.re.kr