120th General Meeting of the KCS

Type Poster Presentation
Area Analytical Chemistry
Room No. Exhibition Hall 2+3
Time 10월 19일 (목요일) 11:00~12:30
Code ANAL.P-195
Subject Phytochemical, Pharmacological and Cytotoxic Characteristics of a Bioactive Compound Isolated from the Aerial Part of Stenochlaena palustris) Bedd
Authors Adhikari Suraj, Wonjae Lee*
College of Pharmacy, Chosun University, Korea
Abstract The ethanolic extract of Stenochlaena palustris Bedd. (Blechnaceae) was investigated for phytochemical, pharmacological and cytotoxic properties in a dose-dependent manner. The gummy concentrate obtained from ethanolic extraction was designed as crude extract with a yield of 17.6% for investigation. Phytochemical study revealed the presence of glycosides, flavonoids, saponines and alkaloids. The antioxidant property was evaluated by both qualitative test (using thin layer chromatography) and quantitative test (DPPH scavenging assay, total phenolic content assay, total flavonoid content assay, total tannin content assay, reducing power assay, nitric oxide scavenging assay, hydrogen peroxide scavenging activity, hydroxyl scavenging activity). In the quantitative assay, the extract showed dose-dependent free radical scavenging activity (IC50 ~30.5µg/mL) which is comparable to that of standard ascorbic acid (IC50 ~7.08 µg/mL). Analgesic activity was investigated on Swiss Albino mice by hot water tail immersion method where extract at dose of 200 mg/kg and 400 mg/kg exhibited significant (P < 0.05 and P < 0.001, respectively) inhibition of pain by 157.27 and 146.15 after 120 and 180 minutes respectively while the standard drug Diclofenac Na inhibition was found to be 217.67 after 180 minutes at a dose of 25 mg/kg body weight. A linear correlation was found between mortality rates of brine shrimp with increased sample concentration in cytotoxicity test. The results tend to suggest that the plant contains active constituent(s) responsible for the mentioned activities and supports the traditional uses of this plant which also require further investigations to isolate bioactive compounds and to identify its underlying mechanisms.
E-mail wlee@chosun.ac.kr