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|
| Type |
Poster Presentation |
| Area |
Analytical Chemistry |
| Room No. |
Exhibition Hall 2+3 |
| Time |
10월 19일 (목요일) 11:00~12:30 |
| Code |
ANAL.P-196 |
| Subject |
Electrochemical immunoassay for amyloid-beta 1?42 peptide in biological fluids interfacing with a gold nanoparticle modified carbon surface |
| Authors |
Hye Jin Lee*, Kyung Min Kim, Suhee Kim
Department of Chemistry, Kyungpook National University, Korea
|
| Abstract |
In this poster, an electrochemical immunosensor involving the formation of a surface sandwich complex on a goldnanoparticle (NP) modified screen printed carbon electrode (SPCE) is demonstrated for the femtomolardetection of amyloid-beta 1–42 peptide(Aβ) in both serum and plasma. Both bioreceptors forming the assay are highly selective antibodies for Aβ, namely antiAβ and which possess different binding sites for the A peptide. In order to improve the sensing performance for complex biological fluidic matrix analysis, different mixed monolayers of thiol modified polyethylene glycol (PEG) and mercaptopropionic acid (MPA) were self-assembled onto the Au NP-SPCE followed by tethering antiA (12F4)to MPA using a heterobifunctional cross linker. |
| E-mail |
4011167@naver.com |
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120th General Meeting of the KCS