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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Exhibition Hall 2+3 |
| Time |
10월 19일 (목요일) 11:00~12:30 |
| Code |
BIO.P-260 |
| Subject |
Effect of ERLBD302-552 on the amyloid fibril formation of α-synuclein |
| Authors |
So young Yoon, Lee Kyunghee*
Department of Chemistry, Sejong University, Korea
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| Abstract |
α-Synuclein (αSyn) protein of Parkinson’s disease patient undergoes conformational change to form amyloid fibrils as insoluble deposit. In our previous study, we have constructed the plasmid ERLBD302-552/pET28 ans overexpressed the His-tagged ERLBD302-552 protein by IPTG induction using BL21(DE3). In the present study, we examined the process of amyloid fibril formation of αSyn in the presence of His-tagged ERLBD302-552 in vitro. We also made the fusion construct ERLBD-L23-αSyn/pET28a by cassette ligation. Finally we discuss the function of ERLBD302-552 on the amyloid fibril formation of αSyn based on the ThT fluorescence kinetics of fusion protein. |
| E-mail |
gee0528@gmail.com |
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120th General Meeting of the KCS