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| Type |
Poster Presentation |
| Area |
Analytical Chemistry |
| Room No. |
Exhibition Hall 2+3 |
| Time |
10월 19일 (목요일) 11:00~12:30 |
| Code |
ANAL.P-200 |
| Subject |
On-Chip Direct Diagnostics based on Grating Coupling of Scattered Nanometals in Evanescent Field Layer |
| Authors |
Seungah Lee, Soyeong Ju1, Suresh Kumar Chakkarapani1, Seong Ho Kang*
Department of Applied Chemistry, Kyung Hee University, Korea
1Department of Chemistry, Kyung Hee University, Korea
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| Abstract |
The grating coupling of a scattered novel nanometals was effectively utilized for direct diagnosis of influenza virus without amplification process. Gold nanopad was used as a substrate for analyzing hybridization of single-stranded DNA (ssDNA) with nanometal tag (silver) by total internal reflection scattering (TIRS) microscopy. The grating separated the scattering signals of the nanotag from that of the substrate and displaced the optical path of the scattered signals to avoid errors caused by numerous acquisitions. This sandwich hybridization based biosensor quantified the target until zeptomolar concentration without interference of background by grating associated TIRS. The detection method is a significant advance in the development of an amplification-free biosensor to greatly reduce the time of sample pretreatment for quantitative screening of influenza A (H7N9) virus. |
| E-mail |
moon1131@gmail.com |
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120th General Meeting of the KCS