120th General Meeting of the KCS

Type Poster Presentation
Area Analytical Chemistry
Room No. Exhibition Hall 2+3
Time 10월 19일 (목요일) 11:00~12:30
Code ANAL.P-201
Subject Analysis of Famphur in Honey by Solid-Phase Extraction and GC-MS
Authors seungho Lee, Hyun-Woo Cho1, Seung Woon Myung*
Department of Chemistry, Kyonggi University, Korea
1Department of Natural Science Chemistry, Kyonggi University, Korea
Abstract Famphur which is a highly hazardous pesticide classified by WHO, may have adverse effects on humans and environments. But there was no guideline for famphur remaining in honey. In this study, a method for the selective determination of famphur in four kinds of honey using solid-phase extraction(SPE) and gas chromatography-mass spectrometry(GC-MS) was established. 5 g of honey was dissolved in 10 mL of water and transferred to a C18 cartridge preconditioned with ethyl acetate and water. After washing with 2 mL of purified water, and then famphur was eluted with 6 mL of ethyl acetate and determined by gas chromatograph with mass spectrometer(GC-MS). Optimization of solid-phase extraction(SPE) parameters was evaluated by the pH of the sample, the type and volume of the elution solvent. Chromatographic separation was achieved on DB-35MS column(30 m × 0.20 mm × 0.33 μm), and oven temperature was raised from 120 ℃ to 310 ℃ at a rate of 25 ℃/min, and then maintained for 2 minutes. The injection mode was the split mode(10:1), and m/z 218.0 was used as the quantitative ions in SIM mode. From the established extraction and GC-MS conditions, the limit of detection(LOD) and limit of quantitation(LOQ) in the spiked sample were 2 ng/g and 5 ng/g, respectively. Recovery studies were performed at 5 ng/g, 10 ng/g, 20 ng/g of fortification levels, and accuracy and precision in working range were 63.7-118.4 % and 1.0-27.7 RSD %, respectively. The calibration curves for the quantitative analysis were obtained the concentration range of 5~50 ng/g with correlation coefficients(R2) from 0.9883 to 0.9958. The proposed method was applied to the analysis of famphur in domestic honey samples.
E-mail seungho0334@hanmail.net