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| Type |
Poster Presentation |
| Area |
Polymer Chemistry |
| Room No. |
Exhibition Hall 2+3 |
| Time |
10월 20일 (금요일) 13:00~14:30 |
| Code |
POLY.P-33 |
| Subject |
3D printing of hydrogel constructs for tissue fabrication |
| Authors |
Giho Choi, Kihoon Kim, Minyoung Kim1, Kwanwoo Shin*
Department of Chemistry, Sogang University, Korea
1Chemistry, Sogang University, Korea
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| Abstract |
Bioprinting is a fabrication method for 3D printing of artificial tissues where living cells are encapsulated in biocompatible materials. Unlike the cell seeding on the premade hydrogel scaffolds, bioprinting enables precise positioning of the cells and microvasculature. Microvasculature is a compulsory part of tissue construct since cellular waste and nutrients are removed and supplied through the microvasculature. However, fabrication of hydrogel constructs with micropores or microvasculature is of a challenge since hydrogel inks are difficult to 3D print precisely. Here, we present several methods to 3D print microporous and microvascular hydrogel constructs. In the first approach, sucrose is added in hydrogel ink to increase the viscosity and the printability, as well as to create micropores which function as microvasculature when leached. Another method includes ionic crosslinking of alginate when 3D printed inside the CaCl2 and Pluronic F127 bath. Pluronic F127 is a shear thinning material which prevents the hydrogel from collapsing during the 3D printing process and is removed afterwards through phase transition. Pluronic F127 can also be used to directly fabricate microvascular structure. Last method includes such microvasculature fabricated by Pluronic F127. Pluronic F127 is 3D printed to make a microvascular structure, and cell-laden hydrogel is poured. When Pluronic F 127 is removed by phase transition, channels devoid of Pluronic F127 function as microvasculature. These methods for fabricating microporous and microvascular structures could improve the material flow between cells and the surrounding hydrogel, promoting the cell viability. |
| E-mail |
gihochoi@sogang.ac.kr |
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120th General Meeting of the KCS