120th General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Exhibition Hall 2+3
Time 10월 19일 (목요일) 11:00~12:30
Code BIO.P-264
Subject Deposition of Single and Multi component ECM and Investigate mechanical properties of ECM vesicles
Authors Minyoung Kim, Kwanwoo Shin1,*
Chemistry, Sogang University, Korea
1Department of Chemistry, Sogang University, Korea
Abstract The extracellular matrix (ECM) has important roles in regulating the development, function and homeostasis of all eukaryotic cells. Essentially, binding of ECM molecules to integrin induces ECM unfolding, thereby spontaneously promoting interactions and fibril assembly. ECM fiber can be coated on GUV by negative charge. Giant unilamellar vesicle (GUV) is a simple model of complex cells. For fibrillogenesis of ECM such as Laminin and Fibronectin outside of vesicle, vesicles were made with PS lipid with negative charge ahead. Then GUV were incubated with ECM dimer at 37℃ during 12hrs. As a result, Single and Multi component ECM coated vesicle was obtained. We analysed structure of binding ECM and investigate mechanical properties by micropipette aspiration. Fibronectin bind themselves by stretching their length and wholly cover on GUV but Laminin bind together by their arms (not stretching) so not evenly cover on GUV. Multi component of ECM will be a good model of cell because there is no distortion and modulus is similar to real cells. Laminin increase area expansion modulus and this GUV can be reached near by real cell’s modulus of mechanical properties.
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