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| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Exhibition Hall 2+3 |
| Time |
10월 19일 (목요일) 11:00~12:30 |
| Code |
BIO.P-266 |
| Subject |
Screening of carbohydrates that elicit immune responses using glycan microarray |
| Authors |
Hyun jiyoung, Injae Shin*
Department of Chemistry, Yonsei University, Korea
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| Abstract |
Lectins expressed on the immune cell surface associate with pathogenic cell surface glycans, the process that leads to stimulation of immune responses to pathogens. Mouse SIGN-R1 (SIGN-related 1) is known to recognize mannose-rich and fucose containing glycans in a Ca2+-dependent manner. When SIGN-R1 binds to glycans on bacteria or viruses, glycan antigens enter cells via lectin-mediated endocytosis to stimulate immune activation. In this study, we applied glycan microarrays containing various glycans to rapidly identify glycans that elicit cell surface lectin-mediated cellular responses. Because binding of glycan ligands to SIGN-R1 on the cell surface leads to production of reactive oxygen species (ROS), the functional glycans on the microarrays are rapidly identified by using a ROS-selective fluorescent probe (PF1). Glycan microarrays were created by attaching 31 unmodified glycans on the hydrazide-coated glass surface. SIGN-R1 expressing cells that were pre-treated with PF1 were applied to the glycan microarrays and the fluorescence intensity of the cells was then determined by using a confocal fluorescence microscopy or a microarray scanner. The results of microarray experiments showed that Man and Fuc possessing glycans stimulate ROS production, a phenomenon that was blocked in the presence of a ROS scavenger or an NADPH oxidase inhibitor. The present study demonstrated that glycan microarrays serve as a useful tool to uncover functional glycans that enhance cell surface lectin-associated cellular responses. |
| E-mail |
hyunjy@yonsei.ac.kr |
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120th General Meeting of the KCS