120th General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Exhibition Hall 2+3
Time 10월 19일 (목요일) 11:00~12:30
Code BIO.P-267
Subject Discovery of Lectin-selective Ligands Using Carbohydrate Library Microarrays
Authors Hyun jiyoung, Injae Shin*
Department of Chemistry, Yonsei University, Korea
Abstract Binding of lectins to glycans, present in cells in the form of glycoconjugates such as glycoproteins, glycolipids and proteoglycans, is a critical process that triggers a number of physiological and pathological events. It is known that lectins have distinctive but overlapping glycan binding properties. To elucidate biological events promoted by lectin binding to glycans, ligands that interact with one lectin but not with other lectins that have overlapping binding properties are in high demand. Ligands with such characterizations can be used as chemical probes to modulate specific glycan-lectin interactions. Plant lectins have been widely employed as biological research tools and diagnostic agents. It has been shown that plant lectins are capable of inducing apoptosis in various cancer cells, even though the molecular mechanism underlying apoptotic cell death remains unclear. Because of their biological and biomedical significance, binding specificities of plant lectins have been extensively examined. In this study we synthesized a library consisting of 55 hydrazide-linked mono- and disaccharide analogs and prepared microarrays containing the analogs. The microarrays were then employed to identify lectin-selective ligands. The microarray study shows that two disaccharide analogs selectively bind to S. tuberosum lectin (STL) and wheat germ agglutinin (WGA). Two compounds in this library were observed to display selective binding to plant lectins and to possess the ability to selectively block binding of the lectins to mammalian cells. The results of cell studies also indicate that two compounds selectively block binding of STL and WGA to mammalian cells unlike a natural ligand LacNAc that suppresses binding of both STL and WGA to cells. This study demonstrates that carbohydrate analog microarrays allow for rapid identification of lectin-selective ligands. Because many plant lectins modulate various biological processes, including apoptosis, it is expected that the method developed in this effort can be utilized to develop ligands that serve as chemical probes to modulate plant lectin-mediated biological processes.
E-mail hyunjy@yonsei.ac.kr