|
Type |
Oral Presentation |
Area |
Oral Presentation of Young Analytical Chemists II |
Room No. |
Room C308+C309 |
Time |
FRI 09:56-: |
Code |
ANAL2.O-21 |
Subject |
Optimization of liquid chromatography mass spectrometry (LC/MS) for the analysis of the ganglioside isomers |
Authors |
Soobin Choi, Sangwon Cha* Department of Chemistry, Hankuk University of Foreign Studies, Korea |
Abstract |
Gangliosides are acidic glycosphingolipids which contain sugar chains with a variety of sialic acid residues. There are hundreds of variations in ganglioside structures based on compositions and structures of oligosaccharide head groups as well as compositions of ceramide cores. However, most LC/MS studies did not include separation of these structural isomers. Therefore, we tried to optimize LC conditions for resolving ganglioside isomers and we specifically focused on developing separation conditions for resolving structural isomers of disialogangliosides, designated to GD1a and GD1b, which are known to be major species in mammal brains. Through optimization processes, we developed an effective LC condition with a conventional C18 column and an appropriate salt additive. And we applied the optimized LC/MS method to the analysis of the total ganglioside extract from porcine brain and successfully analyzed individual isomers of di- and tri- gangliosides. |
E-mail |
csoobin123@gmail.com |
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