120th General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Exhibition Hall 2+3
Time 10월 19일 (목요일) 11:00~12:30
Code BIO.P-273
Subject Development of simple and rapid method for detection of Aichi virus A by Ultra-Fast PCR
Authors WON-CHEOUL JANG *, Youngkwan Kim, Jin-ho KIM, minseon Kim, seunghun jeon, Sang-Hyune Kim, donghyeon Yeo, mingyo Kang, Dayeon Lee1, yerim Lee
Department of Chemistry, Dankook University, Korea
1Department of Molecular biology, Dankook University, Korea
Abstract Aichi viruses has been proposed etiological factors of acute gastroenteritis(GE) that occurs diarrhea and vomiting. Especially, acute gastroenteritis is increasing cause of mortality worldwide in children and the elderly. Therefore, simple and rapid detection for Aichi virus is important for prevention of infection. Previous studies reported that several diagnostic techniques such as RT-PCR, real-time PCR and nested PCR were performed for detecting Aichi virus A. These methods are expensive and time-consuming that require highly precise thermal cycler. For these reasons, amplification techniques for detecting viruses in point-of-care testing(POCT) are required more simple, inexpensive, and rapid than previous studies. Ultra-Fast PCR is simple and movable method that performs 30 cycles within 15 min. In addition, amplification products are observed thorough the window on the top of GENECHECKERTM Ultra-Fast PCR system. A polymer chip for Ultra-Fast PCR is thin and high heat conductive. In this study, we performed detection of Aichi virus A using Ultra-Fast PCR amplification. And we compared Ultra-Fast PCR with previous studies(conventional PCR and real-time PCR). As a result, we used Ultra-Fast PCR to detect Aichi virus A and confirmed that GENECHECKERTM is able to detect Aichi virus within 25 minutes. Furthermore, Ultra-Fast PCR was amplified DNA products 1 hour faster than conventional PCR and real-time PCR. In conclusion, Ultra-Fast PCR has demonstrated simple, rapid, and efficient tool which can be available in molecular diagnosis in POCT.
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