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| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Exhibition Hall 2+3 |
| Time |
10월 19일 (목요일) 11:00~12:30 |
| Code |
BIO.P-276 |
| Subject |
Development of novel antimicrobial peptides derived from Lactophoricin with enhanced antimicrobial activity |
| Authors |
hyunjun Jang, Ji Sun Kim, jiho jung, YONGAE KIM*
Department of Chemistry, Hankuk University of Foreign Studies, Korea
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| Abstract |
Bacteria that have Anti-Microbial Resistance (ARM) are increasing due to antibiotics abuse, nowadays and it has attracted people’s attention to antimicrobial peptides (AMPs). Therefore, we studied LPcin-I and LPcin-II derived from lactophoricin, a cationic amphipathic antimicrobial peptide consisting of 23 amino acids. The antimicrobial activity of alpha helical antimicrobial peptides is controlled by various factors such as net positive charge, α-helicity, and hydrophilic/hydrophobic region. So, we design YK1, YK2 and YK3 derived from LPcin-I . And among these, YK3 showed the best antimicrobial activity so we used it to develop new AMPs with a shorter length and improved antimicrobial activity. First, antimicrobial peptide was mutated to increase the net charge and amphipathicity was reduced to prevent self-aggregation. Finally, mutation to facilitate interaction with the bacterial membrane was done. finally, we designed and selected the new antimicrobial peptides YK5, YK8 and YK11 based on YK3.
Designed all analogues were expressed and purified using several biophysical techniques and characterized using antimicrobial activity tests and various spectroscopic methods like MALDI-TOF MS and CD spectrometry, as well as 1D/2D solution NMR and solid-state NMR techniques in membrane environments.
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| E-mail |
zizidudgh1@naver.com |
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120th General Meeting of the KCS