120th General Meeting of the KCS

Type Award Lecture in Division
Area Recent Progress in Electronic Structure Theory
Room No. Room 208+209+210
Time THU 13:30-:
Code PHYS1-1
Subject Radical-based peptide mass spectrometry and IRMPD spectroscopy
Authors Han Bin Oh
Department of Chemistry, Sogang University, Korea
Abstract Last fifteen years, I have established the biological mass spectrometry laboratory at Sogang University. The main focuses of my research have lied in the development of radical-based peptide mass spectrometry and the IRMPD spectroscopy. In the beginning of my independent career, I have studied how electron capture dissociation mass spectrometry (ECD-MS) worked for the gaseous peptide system and some interesting peptide model systems such as PAMAM dendrimers. Later, we developed a new radical-peptide tandem mass spectrometry method called "free-radical initiated peptides seqencing mass spectrometry (FRIPS MS)". We have demonstrated that FRIPS MS can be a very successful tandem mass spectrometry for peptide seqeuncing, elucidation for post-translational modifications, and top-down mass spectrometry. Towards the goal of establishing FRIPS MS as a practical proteomics tool, we have modified the structures of the FRIPS reagent, enabling a single step peptide sequencing even in positive ion mode. The detailed result will be shown in the symposium. In addition, the structures of gaseous peptide species were probed using IRMPD spectroscopy. Recently, chiral differentiation of L-/ D- amino acids by complexation with a cyclodextrin was demonstrated using IRMPD spectroscopy. Very lately , our laboratory have incorporated artificial intelligence computations and microfluidics technologies into the MS system, which expanded the scope of the mass spectrometry system.
E-mail hanbinoh@sogang.ac.kr