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| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Exhibition Hall 2+3 |
| Time |
10월 19일 (목요일) 11:00~12:30 |
| Code |
BIO.P-278 |
| Subject |
Purification and Inhibition Study of Fatty Acid Synthase, A Potential Target for Anti-cancer drugs |
| Authors |
Jueun Oh, Hyunbeom Lee1,*
Molecular recognition research center, Korea Institute of Science and Technology, Korea
1Molecular Recognition Research Center, Korea Institute of Science and Technology, Korea
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| Abstract |
Fatty acid synthase (FAS) is an enzyme in the palmitate pathway that catabolizes the saturated fatty acid of 16-carbon long palmitate from acetyl-coA and malonyl-coA. Because the fatty acids synthesized in the cancer cells through de novo lipogenesis is important for the cellular membrane synthesis for proliferation, modulation of FAS to control the lipogenesis is a potential therapeutic target for anti-cancer drug candidates.
To study the drug’s efficacy and its mechanism of action against FAS, catalytically active and pure FAS is required. The commercially available FAS may be pure but it is not active thus not appropriate for an activity assay. And the conventionally used crude lysates for a typical enzymatic activity studies lack purity. We have purified FAS from MCF-7 breast cancer and LNCaP prostate cancer cell lines using a method of precipitation, filtration, and using a magnetic particle. Furthermore, in an attempt to discover active compounds against FAS from natural products, we have evaluated the extracts of Glechoma Hederacea L. and Aster Glehni F. Schmidt, natural products known to have lipolysis and antiadipogenic activities, for their capability of inhibiting FAS. |
| E-mail |
dhwndsm@kist.re.kr |
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120th General Meeting of the KCS