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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Exhibition Hall 2+3 |
| Time |
10월 19일 (목요일) 11:00~12:30 |
| Code |
BIO.P-285 |
| Subject |
Structure basis of SAM-dependent methyl transfer reaction catalyzed by enzyme, YrrM |
| Authors |
HUIJEONG RYU, Jungwook Kim*
Department of Chemistry, Gwangju Institute of Science and Technology, Korea
|
| Abstract |
Transfer RNAs (tRNAs) that have proximately 100 nucleotides and ‘clover leaf’ structure play vital role in process of protein synthesis by ribosome. Post-transcriptional modification of RNA affects codon base-pairings and stable folding of tRNA among many types of organism. However, many RNA modification enzymes remain unknown and understanding of the combination between proteins and RNA is limited to the field of RNA modification. Here, we describe the structure and biochemical characterization of YrrM expressed in Escherichia coli, methyltransferase(MTase) family. S-Adenosyl-L-methionine(SAM)-dependent methyltransferases (MTases) are associated with a widely various biological processes including methylation of nucleic acids, proteins, and small molecules using common methyl donor, SAM. The three dimensional structure of Yrrm has been revealed by X-ray crystallography. Finally, we experimentally show enzymatic features of this methyl transfer reaction by in vitro assay. |
| E-mail |
fbgmlwjd94@gist.ac.kr |
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120th General Meeting of the KCS