|
Type |
Poster Presentation |
Area |
Life Chemistry |
Room No. |
Exhibition Hall 2+3 |
Time |
10월 19일 (목요일) 11:00~12:30 |
Code |
BIO.P-288 |
Subject |
Heteroaromatic Sulfones: New Chemical Tools for Selective Detection of Biothiols |
Authors |
Yu Rim Kwon, Chung-Min Park1,* Department of Chemistry, Gangneung-Wonju National University, Korea 1Chemistry, Gangneung-Wonju National University, Korea |
Abstract |
In biological studies, the selective reaction of chemical reagents with protein thiols is very important. This chemical reaction is employed to prevent cysteine-based cross-linking of proteins in common proteomics workflows. In addition, it is applied widely in targeted redox investigations of the mechanisms for understanding physiological and pathological processes in living systems. However, commonly used thiol blocking reagents like iodoacetamide (IAM), N-ethylmaleimide, and others were found to react with protein sulfenic acids (RSOH) which results in many significant errors in biological research. In this presentation, we will report a new heteroaromatic alkylsulfone, 4-(5-methanesulfonyl-[1,2,3,4]tetrazol-1-yl)-phenol (MSTP), as a selective and highly reactive biothiol blocking reagent for biological applications. |
E-mail |
parkc@gwnu.ac.kr |
|