120th General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Exhibition Hall 2+3
Time 10월 19일 (목요일) 11:00~12:30
Code BIO.P-288
Subject Heteroaromatic Sulfones: New Chemical Tools for Selective Detection of Biothiols
Authors Yu Rim Kwon, Chung-Min Park1,*
Department of Chemistry, Gangneung-Wonju National University, Korea
1Chemistry, Gangneung-Wonju National University, Korea
Abstract In biological studies, the selective reaction of chemical reagents with protein thiols is very important. This chemical reaction is employed to prevent cysteine-based cross-linking of proteins in common proteomics workflows. In addition, it is applied widely in targeted redox investigations of the mechanisms for understanding physiological and pathological processes in living systems. However, commonly used thiol blocking reagents like iodoacetamide (IAM), N-ethylmaleimide, and others were found to react with protein sulfenic acids (RSOH) which results in many significant errors in biological research. In this presentation, we will report a new heteroaromatic alkylsulfone, 4-(5-methanesulfonyl-[1,2,3,4]tetrazol-1-yl)-phenol (MSTP), as a selective and highly reactive biothiol blocking reagent for biological applications.
E-mail parkc@gwnu.ac.kr